Recombinant rabbit anti-human CD86 / B7-2 monoclonal antibody, clone DM84

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PRODUCT

  • SKU

    DMR1115-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1115-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target CD86 / B7-2
alias CD86, B7-2, B70, CD28LG2, LAB72, MGC34413
gene id Uniprot ID: P42081
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM84
iso type Rabbit IgG
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, Flow Cytometry
dilution ELISA 1:5000-10000; Flow Cyt 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human B7-2 protein, mFc-His tagged protein binds to Rabbit anti-B7-2 monoclonal antibody (clone: DM84) in a linear range of 1-100 ng/ml.

Flow cytometry analysis of antigen binding of rabbit anti-human B7-2 mAb. (A) The antibody does not bind to 293T cells that do not express B7-2. (B) A clear peak shift of The antibody was seen compared to the control when incubated with B7-2-expressing Daudi cells, indicating strong binding of The antibody to B7-2. Antibodies were incubated at 5 ug/mL.

Flow cytometry analysis of antigen binding of rabbit anti-human B7-2 mAb. (A) The antibody does not bind to 293T cells that do not express B7-2. (B) A clear peak shift of The antibody was seen compared to the control when incubated with B7-2-expressing Raji cells, indicating strong binding of The antibody to B7-2. Antibodies were incubated at 5 ug/mL.

HEK293 cell line transfected with irrelevant protein (A) and human B7-2 (B) were surface stained with Rabbit anti-B7-2 monoclonal antibody 1ug/ml (clone: DM84) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Related Products

PRODUCT

  • SKU

    DMR1115-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1115-500ug

  • Size

    500ug

  • Price

    $2,010

1

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