Recombinant rabbit anti-human EPCAM monoclonal antibody, clone DM147

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PRODUCT

  • SKU

    DMR1175-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1175-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target EPCAM
alias EPCAM, TACSTD1, TROP1, CD326, DIAR5, EGP2, EGP314, EGP40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1:4, KSA, M4S1, MIC18, MK1
gene id Uniprot ID: P16422
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM147
iso type Rabbit IgG
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, Flow Cytometry
dilution ELISA 1:5000-10000; Flow Cyt 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human EPCAM protein, His tagged protein binds to Rabbit anti-EPCAM monoclonal antibody (clone: DM147) in a linear range of 5-100 ng/ml.

EPCAM protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EPCAM (DM147) on Expi293 cells transfected with human EPCAM (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Flow cytometry analysis of antigen binding of Rabbit anti-human EPCAM mAb. (A) The antibody does not bind to Jurkat cells that do not express EPCAM. (B) A clear peak shift of The antibody was seen compared to the control when incubated with EPCAM -expressing HT55 cells, indicating strong binding of The antibody to EPCAM. Antibodies were incubated at 10 ug/mL.

Related Products

PRODUCT

  • SKU

    DMR1175-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1175-500ug

  • Size

    500ug

  • Price

    $2,010

1

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