Recombinant rabbit anti-human L1CAM / CD171 monoclonal antibody, clone DM155

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PRODUCT

  • SKU

    DMR1265-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1265-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target L1CAM / CD171
alias CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1
gene id Uniprot ID: P32004
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM155
iso type Rabbit IgG
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, Flow Cytometry
dilution ELISA 1:5000-10000; Flow Cyt 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate pre-coated by 1 ug/ml (100 ul/well) Human CD171 protein, His tagged protein binds to Rabbit anti-CD171 monoclonal antibody (clone: DM155) in a linear range of 1-100 ng/ml.

CD171 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CD171 (DM155) on Expi293 cells transfected with human CD171 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Flow cytometry analysis of antigen binding of rabbit anti-human CD171 mAb. (A) The antibody does not bind to CHO-S cells that do not express CD171. (B) A clear peak shift of The antibody was seen compared to the control when incubated with CD171-expressing Hela cells, indicating strong binding of The antibody to CD171. Antibodies were incubated at 5 ug/mL.

Related Products

PRODUCT

  • SKU

    DMR1265-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1265-500ug

  • Size

    500ug

  • Price

    $2,010

1

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