Recombinant rabbit anti-human MSLN / Mesothelin monoclonal antibody, clone DM73

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PRODUCT

  • SKU

    DMR1275-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1275-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target MSLN / Mesothelin
alias MSLN, Mesothelin, MPF
gene id Uniprot ID: Q13421
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM73
iso type Rabbit IgG
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, Flow Cytometry, WB
dilution ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human MSLN protein, mFc-His tagged protein binds to Rabbit anti-MSLN monoclonal antibody (clone: DM73) in a linear range of 1-100 ng/ml.

Affinity ranking of different Rabbit anti-MSLN mAb clones by titration of different concentration onto Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody (clone: DM73) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Anti-MSLN antibody at 1/1000 dilution. Lanes: HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution. Predicted band size: 69 kDa, observed band size: 50 kDa

Flow cytometry analysis of antigen binding of rabbit anti-human Mesothelin mAb. (A) The antibody does not bind to 293T cells that do not express Mesothelin. (B) A clear peak shift of The antibody was seen compared to the control when incubated with Mesothelin-expressing Hela cells, indicating strong binding of The antibody to Mesothelin. Antibodies were incubated at 2 ug/mL.

HEK293 cell line transfected with irrelevant protein (A) and human mesothelin (B) were surface stained with Rabbit anti-MSLN monoclonal antibody 1ug/ml ( clone: DM73) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Related Products

PRODUCT

  • SKU

    DMR1275-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1275-500ug

  • Size

    500ug

  • Price

    $2,010

1

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