Recombinant rabbit anti-human SDC1/CD138 monoclonal antibody, clone DM45

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PRODUCT

  • SKU

    DMR1342-100ug

  • Size

    100 ug

  • Price

    $669

1
  • SKU

    DMR1342-500ug

  • Size

    500 ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target SDC1
alias SDC1, Syndecan-1, CD138, SYND1, SDC
gene id Uniprot ID: P18827
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM45
iso type Rabbit IgG
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA IHC FC
dilution ELISA 1:5000-10000; Flow Cyt 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Flow cytometry analysis of antigen binding of rabbit anti-human CD138 mAb . (A) the antibody does not bind to Jurkat cells that do not express CD138. (B) A clear peak shift of the antibody was seen compared to the control when incubated with CD138-expressing MM.1S cells, indicating strong binding of the antibody to CD138. Antibodies were incubated at 5 ug/mL.

Expi 293 cell line transfected with irrelevant protein (A) and human CD138 (B) were surface stained with Rabbit anti-CD138 monoclonal antibody 1ug/ml (clone: DM45) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

the antibody at 5ug/ml staining CD138 in RPMI-8226 MM xenografts in NSG mice by IHC ;


PRODUCT

  • SKU

    DMR1342-100ug

  • Size

    100 ug

  • Price

    $669

1
  • SKU

    DMR1342-500ug

  • Size

    500 ug

  • Price

    $2,010

1