Recombinant rabbit anti-human SLAMF6 / NTB-A monoclonal antibody, clone DM159

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PRODUCT

  • SKU

    DMR1352-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1352-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target SLAMF6 / NTB-A
alias NTB-A, SLAMF6, Ly108, NK-T-B-antigen, CD352, KALI
gene id Uniprot ID: Q96DU3
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM159
iso type Rabbit IgG
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, Flow Cytometry, WB, IHC
dilution ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000; IHC 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Flow cytometry analysis with Anti-NTB-A (DM159) on Expi293 cells transfected with human NTB-A (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Anti-NTB-A antibody at 1/1000 dilution. Lanes: RAJI (human Burkitt's lymphoma B lymphocyte), whole cell lysate. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution. Predicted band size: 37 kDa, observed band size: 60 kDa

DMR1352 at 10 ug/ml staining NTB-A in Raji cells by IHC

Flow cytometry analysis of antigen binding of rabbit anti-human NTB-A mAb. (A) The antibody does not bind to 293T cells that do not express NTB-A. (B) A clear peak shift of The antibody was seen compared to the control when incubated with NTB-A-expressing Raji cells, indicating strong binding of The antibody to NTB-A. Antibodies were incubated at 5 ug/mL.

Flow cytometry analysis of antigen binding of rabbit anti-human NTB-A mAb. (A) The antibody does not bind to 293T cells that do not express NTB-A. (B) A clear peak shift of The antibody was seen compared to the control when incubated with NTB-A-expressing Jurkat cells, indicating strong binding of The antibody to NTB-A. Antibodies were incubated at 5 ug/mL.

Related Products

PRODUCT

  • SKU

    DMR1352-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1352-500ug

  • Size

    500ug

  • Price

    $2,010

1

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