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DMS1027-100ug
100 ug
$350
| target | CD274 |
| alias | PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1 |
| gene id | Uniprote ID: Q9NZQ7 |
| host species | Humanized |
| reactivity | Human |
| iso type | IgG1 |
| formulation | Lyophilized from sterile PBS, pH 7.4. Typically, 5% - 8% trehalose is added as a protective agent prior to lyophilization. |
| reconstitution | Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 minutes with occasional gentle tapping. It is recommended that 0.1% HSA be added as a carrier protein for long-term storage. |
| applications | ELISA; Flow Cytometry |
| dilution | ELISA 1:5000-10000; Flow Cyt 1:100 |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months. |
| note | Other commonly used names in publications refering to this antibody: MPDL3280A, RG7446 |
ELISA plate coated with 2 ug/ml (100 ul/well) Human PDL1, mFc-His tagged protein binds to anti-PDL1 neutralizing antibody in a linear range of 0.24-7.81 ng/ml.
HEK293 cell line transfected with irrelevant protein (B) and human PDL1 (A) were surface stained with anti-PDL1 neutralizing antibody 1ug/ml (atezolizumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.
DMS1027-100ug
100 ug
$350