Recombinant FGF2 protein contains 154 amino acids. It is a 17 kDa non-glycosylated monomer. This is a "thermostable" version of FGF2 protein specially engineered to enhance its thermostability without affecting its biological functions. This protein exibited superior stability in culture medium and is ideal for use as a component of cell culture media for stem cell research.
|alias||FGF2, Fibroblast growth factor 2, BFGF, FGF-2, FGFB, HBGF-2, Fibroblast Growth Factor-basic|
|production host||HEK293 Cells|
|purity||Greater than 95% as determined by reducing SDS-PAGE.|
|endotoxin||Typically ≤ 0.5 ng/mL EC50|
|formulation||The protein was lyophilized after filtrated through a 0.22 μm filter. Before lyophilization, the protein is in PBS buffer.|
|reconstitution||Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA be added as a carrier protein for long term storage.|
|shipping||Ship at ambient conditions.|
|storage||Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.|
SDS-Page analysis of FGF2
Activity Measurement: determined by dose-dependent stimulation of proliferation of 3T3 cells.