This recombinant FGF7 protein was purifed from the supernant of an engineered HEK293 stable cell line cultured in chemically defined serum-free media. The protein exhibits high activity and high stability due to natural conformation and native glycosylation pattern. Batch-to-batch variation is negligible because the protein is produced by a stable cell line and not by transiently-transfected heterogeneous cells. This protein contains NO Tags. It is 100% natural and is endotoxin-free. It is a monomeric glycoprotein with an apparent molecular mass of 17 and 30 kDa.
|alias||KGF, HBGF-7, fibroblast growth factor 7|
|production host||HEK293 Stable Cell Line|
|purity||Greater than 95% as determined by reducing SDS-PAGE.|
|endotoxin||Typically ≤ 0.5 ng/mL EC50|
|formulation||The protein was lyophilized after filtrated through a 0.22 μm filter. Before lyophilization, the protein is in PBS buffer.|
|reconstitution||Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA be added as a carrier protein for long term storage.|
|shipping||Ship at ambient conditions.|
|storage||Lyophilized form is stable at room temperature for 3 weeks. Keep at -20°C for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.|
SDS-PAGE Analysis of FGF7
Activity analysis: Activity was determined by dose-dependent stimulation of 4MBr-5 cell proliferation.