Mouse anti-Human ALB Monoclonal Antibody (Clone KT11)

ELISA: Microtiter wells were coated with KT11 at 5 ug/mL as the capture antibody. Human ALB was used as the antigen. Peroxidase conjugated mouse anti-human ALB monoclonal antibody (KT32) was used as the detection antibody. Result: KT11 and KT32 can be used as a matched antibody pair to detect and quantify the concentration of human ALB.

Immunohistochemistry: IHC-P analysis of liver tissue by ALB antibody (KT11). IHC-P was performed using sections of the formalin-fixed paraffin-embedded liver tissue.

Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug KT11 with Hep G2 lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. ALB (KT11) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Hep G2 lysate Lane 2: ALB immunoprecipitated from Hep G2 lysate by KT11 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: KT11 can immunoprecipitate ALB.

Western Blot: 15 ug of human plasma was run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. KT11 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. ALB band was visualized using ECL Substrate. Result: KT11 can detect human ALB by Western blotting.