Home/ Products / Mouse Monoclonal Abs
WZA1978-100ul
100uL
$505
| target | NEDD9 |
| host | Mouse |
| gene id | 4739 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K70047_16B6 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K70047_16B6 with U-251MG lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. NEDD9 (K70047_16B6) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: U-251MG lysate Lane 2: NEDD9 immunoprecipitated from U-251MG lysate by K70047_16B6 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K70047_16B6 can immunoprecipitate NEDD9.
Western Blot: 15 ug of U-251MG lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K70047_16B6 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. NEDD9 band was visualized using ECL Western Blotting Substrate. Result: K70047_16B6 can detect NEDD9 by Western blotting.
WZA1978-100ul
100uL
$505