Rat anti-Human FGA Monoclonal Antibody (Clone KT9)

ELISA: Microtiter wells were coated with KT9 at 5 ug/mL as the capture antibody. Human FGA was used as the antigen. Peroxidase conjugated rat anti-human FGA monoclonal antibody (KT10) was used as the detection antibody. Result: KT9 and KT10 can be used as a matched antibody pair to detect and quantify the concentration of FGA.

Immunohistochemistry: IHC-P analysis of placenta tissue by FGA antibody (KT9). IHC-P was performed using sections of the formalin-fixed paraffin-embedded placenta tissue.

Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug KT9 with plasma containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. FGA (KT9) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti- mouse light chain specific IgG (which cross reacts with rat IgG) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: plasma Lane 2: FGA immunoprecipitated from plasma by KT9 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: KT9 can immunoprecipitate FGA.

Western Blot: 15 ug of human plasma was run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. KT9 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-rat IgG was used as the secondary antibody. FGA band was visualized using ECL Substrate. Result: KT9 can detect human FGA by Western blotting.