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WZA2244-100ul
100uL
$540
| target | HNMT |
| host | Mouse |
| gene id | 3176 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K40053_20E2 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K40053_20E2 with Hep G2 lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. HNMT (K40053_20E2) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Hep G2 lysate Lane 2: HNMT immunoprecipitated from Hep G2 lysate by K40053_20E2 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K40053_20E2 can immunoprecipitate HNMT.
Western Blot: 15 ug of Hep G2 lysate and 15 ug of mouse brain tissue lysate were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K40053_20E2 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. HNMT band was visualized using ECL Western Blotting Substrate. Lane 1: 15 ug of Hep G2 lysate Lane 2: 15 ug of mouse brain tissue lysate Result: K40053_20E2 can detect HNMT by Western blotting.
WZA2244-100ul
100uL
$540