Mouse anti-Human PML Monoclonal Antibody (Clone K1E038_11H9)

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PRODUCT

  • SKU

    WZA2448-100ul

  • Size

    100uL

  • Price

    $540

1
This antibody was raised against a protein with Unprot Accession # P29590. It's core sequence is: EAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNETQKISQLAAV
target PML
host Mouse
gene id 5371
reactivity Human
clonality Monoclonal
clone number K1E038_11H9
iso type IgG1
conjugate Unconjugated
applications IHC, ICC, IP
formulation Supplied as solution in phosphate buffered saline containing 0.09% sodium azide
dilution IHC 1:200, IP 1:100
shipping Ship at ambient conditions or with ice packs.
storage Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

Images

Immunohistochemistry: IHC-P analysis of gall bladder tissue by PML antibody (K1E038_11H9). IHC-P was performed using sections of the formalin-fixed paraffin-embedded gall bladder tissue.

Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K1E038_11H9 with HeLa lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. PML (K1E038_9D2) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HeLa lysate Lane 2: PML immunoprecipitated from HeLa lysate by K1E038_11H9 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K1E038_11H9 can immunoprecipitate PML.


PRODUCT

  • SKU

    WZA2448-100ul

  • Size

    100uL

  • Price

    $540

1