Mouse anti-Phospho Tyrosine Monoclonal Antibody (Clone K06352_8E1)

Dot blotting: Various peptides were loaded on nitrocellulose membrane. The membrane was blocked with 5% non-milk. K06352_8E1 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lane 1: pTyr peptides library Lane 2: pThr peptides library Lane 3: pSer peptides library Lane 4: Tyr peptides library Lane 5: Thr peptides library Lane 6: Ser peptides library Result: K06352_8E1 can detect phospho tyrosine peptides by dot blotting.

Western Blot: HeLa cells treated with or without 1 mM sodium vanadate for 45 min. Lysates were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K06352_8E1 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Proteins with phospho tyrosine modifications were visualized using ECL Western Blotting Substrate. Lane 1: 15 ug of HeLa lysate Lane 2: 15 ug of sodium vanadate treated HeLa lysate Result: K06352_8E1 can detect proteins with phospho tyrosine modification by Western blotting.