Mouse anti-Human TP53 Monoclonal Antibody (Clone K40057_16A9)

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PRODUCT

  • SKU

    WZA2600-100ul

  • Size

    100uL

  • Price

    $540

1
This antibody was raised against a protein with Unprot Accession # P04637. It's core sequence is: PGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGP
target TP53
host Mouse
gene id 7157
reactivity Human
clonality Monoclonal
clone number K40057_16A9
iso type IgG1
conjugate Unconjugated
applications IHC, ICC, IP
formulation Supplied as solution in phosphate buffered saline containing 0.09% sodium azide
dilution IHC 1:200, IP 1:100
shipping Ship at ambient conditions or with ice packs.
storage Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

Images

Immunohistochemistry: IHC-P analysis of skin tissue by TP53 antibody (K40057_16A9). IHC-P was performed using sections of the formalin-fixed paraffin-embedded skin tissue.

Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K40057_16A9 with HEK-293 lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. TP53 (K40057_2A3) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HEK-293 lysate Lane 2: TP53 immunoprecipitated from HEK-293 lysate by K40057_16A9 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K40057_16A9 can immunoprecipitate TP53.


PRODUCT

  • SKU

    WZA2600-100ul

  • Size

    100uL

  • Price

    $540

1