Home/ Products / Anti-Tag and Internal Reference Antibodies
WZA2625-100ul
100uL
$540
| target | V5 Tag |
| host | Mouse |
| clonality | Monoclonal |
| clone number | K05002_3F6 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IP, WB, IF |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:3000-1:4000, IP 1:100, IF 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunofluorescence analysis of HEK-293 cells either transfected with a construct expressing V5 tagged ARF3 (Cat. P61801QFTN) at the N-terminus (A) or untransfected (B). The cells were fixed, permeabilized, blocked and incubated with 10 ug/ml of K05002_3F6 as the primary antibody. FITC-labeled goat anti-mouse IgG antibody (green) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). Result: K05002_3F6 can detect V5 tag by Immunofluorescence.
Immunofluorescence analysis of HEK-293 cells either transfected with a construct expressing V5 tagged CDA (Cat. PQ-621) at the C-terminus (A) or untransfected (B). The cells were fixed, permeabilized, blocked and incubated with 10 ug/ml of K05002_3F6 as the primary antibody. FITC-labeled goat anti-mouse IgG antibody (green) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). Result: K05002_3F6 can detect V5 tag by Immunofluorescence.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K05002_3F6 with 200 ug of lysate extracted from HEK-293 transfected with V5 tagged ARF3 at N-terminus. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag protein (K05002_10F7) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HEK-293 lysate (transfected with V5 tagged ARF3 (Cat. P61801QFTN) at N-terminus) Lane 2: V5 tagged protein immunoprecipitated by KT25 from the same lysate as Lane 1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K05002_3F6 can immunoprecipitate V5 tag.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K05002_3F6 with 200 ug of lysate extracted from HEK-293 transfected with V5 tagged CDA at C-terminus. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag antibody (K05002_10F7) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HEK-293 lysate (transfected with V5 tagged CDA (Cat. PQ-621) at C-terminus) Lane 2: V5 tagged protein immunoprecipitated by KT25 from the same lysate as Lane 1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K05002_3F6 can immunoprecipitate V5 tag.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K05002_3F6 with untransfected HEK-293 lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. V5 tag (K05002_3F6) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Untransfected HEK-293 lysate Lane 2: Sample immunoprecipitated from untransfected HEK-293 lysate by KT25 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: Unspecific immunoprecipitation by K05002_3F6 was not obsearved.
Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K05002_3F6 at 0.25 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. V5 tagged protein bands were visualized using ECL Western Blotting Substrate. Lane 1: 7.5 ug of HEK-293 lysate (untransfected) Lane 2: 7.5 ug of HEK-293 lysate (transfected with a construct expressing V5 tagged CDA (Cat. PQ-621) at C-terminus) Lane 3: 7.5 ug of HEK-293 lysate (transfected with a construct expressing V5 tagged ARF3 (Cat. P61801QFTN) at N-terminus) Result: K05002_3F6 can detect V5 tag by Western blotting.
WZA2625-100ul
100uL
$540