Mouse anti-Human TRIM25 Monoclonal Antibody (Clone K16262_13C1)

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PRODUCT

  • SKU

    WZA2635-100ul

  • Size

    100uL

  • Price

    $540

1
This antibody was raised against a protein with Unprot Accession # Q14258. It's core sequence is: LSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDP
target TRIM25
host Mouse
gene id 7706
reactivity Human
clonality Monoclonal
clone number K16262_13C1
iso type IgG1
conjugate Unconjugated
applications IP
formulation Supplied as solution in phosphate buffered saline containing 0.09% sodium azide
dilution IP 1:100
shipping Ship at ambient conditions or with ice packs.
storage Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

Images

Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K16262_13C1 with A549 lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. TRIM25 (K16262_12G6) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: A549 lysate Lane 2: TRIM25 immunoprecipitated from A549 lysate by K16262_13C1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K16262_13C1 can immunoprecipitate TRIM25.


PRODUCT

  • SKU

    WZA2635-100ul

  • Size

    100uL

  • Price

    $540

1