Home/ Products / Mouse Monoclonal Abs
WZA2647-100ul
100uL
$540
| target | TSPAN7 |
| host | Mouse |
| gene id | 7102 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | KAA131_7G2 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:200, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunohistochemistry: IHC-P analysis of pancreas tissue by TSPAN7 antibody (KAA131_7G2). IHC-P was performed using sections of the formalin-fixed paraffin-embedded pancreas tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of KAA131_7G2 with HEK-293 lysate (transfected with TSPAN7) containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. TSPAN7 (K06373_16D1) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HEK-293 lysate (transfected with TSPAN7) Lane 2: TSPAN7 immunoprecipitated from HEK-293 lysate (transfected with TSPAN7) lysate by KAA131_7G2 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: KAA131_7G2 can immunoprecipitate TSPAN7.
WZA2647-100ul
100uL
$540