Rat anti-Mouse Mapre2 Monoclonal Antibody (Clone KT52)

Immunohistochemistry: IHC-P analysis of mouse brain tissue by anti-Mapre2 antibody (KT52). IHC-P was performed using sections of the formalin-fixed paraffin-embedded mouse brain tissue.

Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug KT52 with rat brain tissue lysate containing 200 ug total protein. After absorption with Protein G beads the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-Mapre2 (KT52) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse light chain specific IgG (which cross reacts with rat IgG) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Rat brain tissue lysate Lane 2: Mapre2 immunoprecipitated from rat brain tissue lysate by KT52 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: KT52 can immunoprecipitate Mapre2.

Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT52 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-rat IgG was used as the secondary antibody. Mapre2 band was visualized using ECL Western Blotting Substrate. Lane 1: 15 ug of rat brain tissue lysate Lane 2: 15 ug of mouse brain tissue lysate Lane 3: 15 ug of NIH-3T3 lysate Lane 4: 15 ug of PMA treated Jurkat lysate Result: KT52 can detect Mapre2 by Western blotting.