Home/ Products / Mouse Monoclonal Abs
WZA2902-100ul
100uL
$540
| target | DCBLD2 |
| host | Mouse |
| gene id | 131566 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | KAA158_5A11 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of KAA158_5A11 with PC-3 lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. DCBLD2 (KAA158_5A11) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: PC-3 lysate Lane 2: DCBLD2 immunoprecipitated from PC-3 lysate by KAA158_5A11 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: KAA158_5A11 can immunoprecipitate DCBLD2.
Western Blot: 15 ug of PC-3 lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KAA158_5A11 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. DCBLD2 band was visualized using ECL Western Blotting Substrate. Result: KAA158_5A11 can detect DCBLD2 by Western blotting.
WZA2902-100ul
100uL
$540