Home/ Products / Mouse Monoclonal Abs
WZA2932-100ul
100uL
$795
| target | KRT14 |
| host | Mouse |
| gene id | 3861 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K16228_8G11 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:500, WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunohistochemistry: IHC-P analysis in esophageal squamous cell carcinoma by KRT14 antibody (K16228_8G11). IHC-P was performed using sections of the formalin-fixed paraffin-embedded esophageal squamous cell carcinoma tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K16228_8G11 with A-431 lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. KRT14 (K16228_8G11) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: 15 ug of A-431 lysate Lane 2: KRT14 immunoprecipitated from A-431 lysate by K16228_8G11 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K16228_8G11 can immunoprecipitate KRT14.
Western Blot: 15 ug of A-431 lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K16228_8G11 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. KRT14 band was visualized using ECL Substrate. Result: K16228_8G11 can detect KRT14 by Western blotting.
WZA2932-100ul
100uL
$795