Home/ Products / Mouse Monoclonal Abs
WZA3059-100ul
100uL
$540
| target | RNF19B |
| host | Mouse |
| gene id | 127544 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K29064_5H7 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:200-1:250, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunohistochemistry: IHC-P analysis of testis tissue by RNF19B antibody (K29064_5H7). IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K29064_5H7 with 200 ug of cell lysate extracted from HEK-293 transfected with 6XHis tagged RNF19B at N-terminus. After absorption with Protein G beads the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-His tag antibody (K56006_9D10) at 0.5 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HEK-293 lysate (transfected with 6XHis tagged RNF19B (Cat. C469AKZG0-192) at N-terminus) Lane 2: RNF19B immunoprecipitated by K29064_5H7 from the same lysate as Lane 1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K29064_5H7 can immunoprecipitate RNF19B.
WZA3059-100ul
100uL
$540