Home/ Products / Mouse Monoclonal Abs
WZA3329-100ul
100uL
$540
| target | RNF8 |
| host | Mouse |
| gene id | 9025 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K92039_15B9 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K92039_15B9 with 200 ug of cell lysate extracted from HEK-293 transfected with 6XHis tagged RNF8 at C-terminus. After absorption with Protein G beads the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-His tag antibody (K56006_9D10) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HEK-293 lysate (transfected with 6XHis tagged RNF8 (Cat. P69874QA) at C-terminus) Lane 2: RNF8 immunoprecipitated by K92039_15B9 from the same lysate as Lane 1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K92039_15B9 can immunoprecipitate RNF8.
WZA3329-100ul
100uL
$540