Home/ Products / Mouse Monoclonal Abs
WZA3570-100ul
100uL
$540
| target | DCLK3 |
| host | Mouse |
| gene id | 85443 |
| reactivity | Human, Mouse |
| clonality | Monoclonal |
| clone number | K56039_13F6 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K56039_13F6 with mouse testis tissue lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. DCLK3 (K56039_13F6) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Mouse testis tissue lysate Lane 2: DCLK3 immunoprecipitated from mouse testis tissue lysate by K56039_13F6 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K56039_13F6 can immunoprecipitate DCLK3.
Western Blot: 15 ug of mouse testis tissue lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K56039_13F6 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. DCLK3 band was visualized using ECL Western Blotting Substrate. Result: K56039_13F6 can detect DCLK3 by Western blotting.
WZA3570-100ul
100uL
$540