Home/ Products / Mouse Monoclonal Abs
WZA3857-100ul
100uL
$540
| target | SKP2 |
| host | Mouse |
| gene id | 6502 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K2K010_4G2 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:100-1:200, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunohistochemistry: IHC-P analysis of breast cancer tissue by SKP2 antibody (K2K010_4G2). IHC-P was performed using sections of the formalin-fixed paraffin-embeddded breast cancer tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K2K010_4G2 with Caco-2 lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. SKP2 (K2K006_5D8) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Caco-2 lysate Lane 2: SKP2 immunoprecipitated from Caco-2 lysate by K2K010_4G2 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K2K010_4G2 can immunoprecipitate SKP2.
WZA3857-100ul
100uL
$540