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WZA3873-100ul
100uL
$540
| target | HSD11B1 |
| host | Mouse |
| gene id | 3290 |
| reactivity | Human, Mouse |
| clonality | Monoclonal |
| clone number | K9N028_1E4 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K9N028_1E4 with mouse liver tissue lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. HSD11B1 (OC-627) at 0.5 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Mouse liver tissue lysate Lane 2: HSD11B1 immunoprecipitated from mouse liver tissue lysate by K9N028_1E4 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K9N028_1E4 can immunoprecipitate HSD11B1.
WZA3873-100ul
100uL
$540