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WZA4128-100ul
100uL
$795
| target | BCL2 |
| host | Mouse |
| gene id | 596 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | KAA270_3G8 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:500, WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunohistochemistry: IHC-P analysis of breast cancer tissue by BCL2 antibody (KAA270_3G8). IHC-P was performed using sections of the formalin-fixed paraffin-embedded breast cancer tissue.
Immunohistochemistry: IHC-P analysis of T-cell lymphoma tissue by BCL2 antibody (KAA270_3G8). IHC-P was performed using sections of the formalin-fixed paraffin-embedded T-cell lymphoma tissue.
Immunohistochemistry: IHC-P analysis of thyroid cancer tissue by BCL2 antibody (KAA270_3G8). IHC-P was performed using sections of the formalin-fixed paraffin-embedded thyroid cancer tissue.
Immunohistochemistry: IHC-P analysis of tonsil tissue by BCL2 antibody (KAA270_3G8). IHC-P was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug KAA270_3G8 with THP-1 lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto PVDF membrane. BCL2 (KAA270_3G8) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: THP-1 lysate Lane 2: BCL2 immunoprecipitated from THP-1 lysate by KAA270_3G8 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody. Result: KAA270_3G8 can immunoprecipitate BCL2.
Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KAA270_3G8 at 1 ug/mL was used as the primary antibody and peroxidase-conjugated goat anti-mouse IgG was used as the secondary antibody. BCL2 band was visualized using ECL Substrate. Lane 1: 15 ug of U-937 lysate Lane 2: 15 ug of Jurkat lysate Lane 3: 15 ug of THP-1 lysate Result: KAA270_3G8 can detect BCL2 by Western blotting.
WZA4128-100ul
100uL
$795