Home/ Products / Anti-Tag and Internal Reference Antibodies
WZA4130-100ul
100uL
$505
| target | GST Tag |
| host | Rat |
| clonality | Monoclonal |
| clone number | K01004_28F1 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:2000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K01004_28F1 with 200 ug of cell lysate extracted from HEK-293 transfected with GST tagged ARF3 at N-terminus. After absorption with Protein G beads,the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. GST tag (K01003_17B11) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HEK-293 lysate (transfected with GST tagged ARF3 (Cat. P61801QDGH) at N-terminus) Lane 2: GST tagged protein immunoprecipitated by K01004_28F1 from the same lysate as Lane 1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K01004_28F1 can immunoprecipitate GST tag.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K01004_28F1 with 200 ug of cell lysate extracted from HEK-293 transfected with GST tagged CDA at C-terminus. After absorption with Protein G beads the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. GST tag (K01003_17B11) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HEK-293 lysate (transfected with GST tagged CDA (Cat. P60351QDGH) at C-terminus) Lane 2: GST tagged protein immunoprecipitated by K01004_28F1 from the same lysate as Lane 1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K01004_28F1 can immunoprecipitate GST tag.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K01004_28F1 with untransfected HEK-293 lysate containing 200 ug of total protein. After absorption with Protein G beads,the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. GST tag (K01003_17B11) at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Untransfected HEK-293 lysate Lane 2: Sample immunoprecipitated from untransfected HEK-293 lysate by K01004_28F1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: Unspecific immunoprecipitation by K01004_28F1 was not observed.
Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K01004_28F1 at 0.5 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. GST tagged protein bands were visualized using ECL Western Blotting Substrate. Lane 1: 7.5 ug of HEK-293 lysate (untransfected) Lane 2: 7.5 ug of HEK-293 lysate (transfected with a construct expressing GST tagged CDA (Cat. P60351QDGH) at C-terminus) Lane 3: 7.5 ug of HEK-293 lysate (transfected with a construct expressing GST tagged ARF3 (Cat. P61801QDGH) at N-terminus) Result: K01004_28F1 can detect GST tag by Western blotting.
WZA4130-100ul
100uL
$505