Home/ Products / Rat Monoclonal Abs
WZA4180-100ul
100uL
$650
| target | Clasp2 |
| reactivity | Mouse, Human |
| host | Rat |
| clonality | Monoclonal |
| clone number | KT68 |
| iso type | IgG2b |
| conjugate | Unconjugated |
| applications | IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug KT68 with HeLa lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. CLASP2 (KT68) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HeLa lysate Lane 2: CLASP2 immunoprecipitated from HeLa lysate by KT68 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: KT68 can immunoprecipitate CLASP2.
Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT68 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-rat IgG was used as the secondary antibody. Clasp2 band was visualized using ECL Substrate.Lane 1: 15 ug of HEK-293 lysateLane 2: 15 ug of Jurkat lysateLane 3: 15 ug of HeLa lysate Result: KT68 can detect Clasp2 by Western blotting.
WZA4180-100ul
100uL
$650