Mouse anti-Human MSH2 Monoclonal Antibody (Clone K52012_12H5)

Immunohistochemistry: IHC-P analysis of tonsil tissue by MSH2 antibody (K52012_12H5). IHC-P was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue.

Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K52012_12H5 with HEK-293 lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. MSH2 (K52012_5C3) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HEK-293 lysate Lane 2: MSH2 immunoprecipitated from HEK-293 lysate by K52012_12H5 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K52012_12H5 can immunoprecipitate MSH2.

Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K52012_12H5 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. MSH2 band was visualized using ECL Western Blotting Substrate. Lane 1: 15 ug of HeLa lysate Lane 2: 15 ug of HEK-293 lysate Lane 3: 15 ug of A549 lysate Lane 4: 15 ug of A-375 lysate Result: K52012_12H5 can detect MSH2 by Western blotting.