Mouse anti-Human KHK Monoclonal Antibody (Clone K52018_1E9)

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PRODUCT

  • SKU

    WZA4239-100ul

  • Size

    100uL

  • Price

    $795

1
This antibody was raised against a protein with Unprot Accession # P50053. It's core sequence is: MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSMAPGHVADFLVADFRRRGVDVSQVAWQSKGDTPSSCCIINNSNGNRTIVLHDTSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV
target KHK
host Mouse
gene id 3795
reactivity Human, Rat
clonality Monoclonal
clone number K52018_1E9
iso type IgG2b
conjugate Unconjugated
applications IHC, ICC, IP
formulation Supplied as solution in phosphate buffered saline containing 0.09% sodium azide
dilution IHC 1:200, IP 1:100
shipping Ship at ambient conditions or with ice packs.
storage Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

Images

Immunohistochemistry: IHC-P analysis of liver tissue by KHK antibody (K52018_1E9). IHC-P was performed using sections of the formalin-fixed paraffin-embedded liver tissue.

Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K52018_1E9 with rat kidney tissue lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti- KHK (K52018_1E9) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: Rat kidney tissue lysate Lane 2: KHK immunoprecipitated from rat kidney tissue lysate by K52018_1E9 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K52018_1E9 can immunoprecipitate KHK.


PRODUCT

  • SKU

    WZA4239-100ul

  • Size

    100uL

  • Price

    $795

1