Home/ Products / Mouse Monoclonal Abs
WZA4732-100ul
100uL
$720
| target | ARAF |
| host | Mouse |
| gene id | 369 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K40035_7G7 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K40035_7G7 with K-562 lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. ARAF (K40035_13G8) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: K-562 lysate Lane 2: ARAF immunoprecipitated from K-562 lysate by K40035_7G7 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K40035_7G7 can immunoprecipitate ARAF.
WZA4732-100ul
100uL
$720