Home/ Products / Mouse Monoclonal Abs
WZA5412-100ul
100uL
$540
| target | BRAF V600E |
| host | Mouse |
| gene id | 673 |
| reactivity | Human |
| clonality | Oligoclonal |
| iso type | IgG |
| conjugate | Unconjugated |
| applications | IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of OC-176 with A-375 lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti-BRAF monoclonal antibody was used as the primary antibody and peroxidase conjugated goat anti-rabbit IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: A-375 lysate Lane 2: BRAF immunoprecipitated from A-375 lysate by OC-176 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Lane 4: U-251MG lysate Lane 5: BRAF immunoprecipitated from U-251MG lysate by OC-176 Lane 6: The same as Lane 5 but KT82 was used as IgG isotype control antibody Result: OC-176 can specifically immunoprecipitate BRAF with V600E point mutation while cannot immunoprecipitate wildtype BRAF.
WZA5412-100ul
100uL
$540