Home/ Products / Mouse Monoclonal Abs
WZA5987-100ul
100uL
$580
| target | PCNA |
| host | Human |
| gene id | 5111 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K29023_14A4 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K29023_14A4 with MOLT-4 lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. PCNA (K29023_7F12) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: MOLT-4 lysate Lane 2: PCNA immunoprecipitated from MOLT-4 lysate by K29023_14A4 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K29023_14A4 can immunoprecipitate PCNA.
Indirect ELISA96-well microtitre plates were coated with 1 ug/mL of PCNA protein at 4 °C overnight. Plates were blocked with 2 % BSA in PBS-T (0.1 % Tween) at room temperature for 1 hour. Serial diluted of K29023_14A4 was used as the primary antibody and added into the plates for 1 hour incubation. Plates were washed with PBS-T for three times and then incubated with HRP conjugated Human IgG1 for 1 hour. After washed with PBS-T for three times, the plates were incubated with ABTS for 15 minute. The absorbance (optical density) was read with a plate reader.
WZA5987-100ul
100uL
$580