Recombinant Rabbit anti-Human SMAD3 Monoclonal Antibody (Clone K01_1L29)

PRODUCT

This antibody was raised against a protein with Unprot Accession # P84022. It's core sequence is: AGSPNLSPNPMSPAHNNL

formulation	50mM Tris-Glycine(pH 7.4),0.15M NaCl,40% Glycerol,0.01% Sodium azide and 0.05% BSA
applications	IHC, ICC, WB
dilution	IHC 1:50-1:100, WB 1:500-1:1000
shipping	Ship at ambient conditions or with ice packs.
storage	Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

Immunocytochemistry analysis of Smad3 (green) in A549 using Smad3 antibody,and DAPI(blue).

Immunohistochemistry: Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Smad3 antibody.

Western Blot: Western blot analysis of Smad3 in Jurkat,C6,Hela lysates using Smad3 antibody.