Biotinylated human CD80 / B7-1 (Val35-Asn242) protein, with C-Fc-Avi Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1206-25ug

  • Size

    25ug

  • Price

    $525

1
  • SKU

    YCD1206-200ug

  • Size

    200ug

  • Price

    $2,070

1
This recombinant protein contains Val35-Asn242 of UniProt P33681. It has a human IgG1 Fc tag at the C-terminus, followed by an Avi tag. The protein's calculated molecular weight is 52.0 kDa, migrating at 65-80 kDa in reducing SDS-PAGE due to glycosylation.
gene name CD80
gene id UniProt P33681
host species Human
production host HEK293
tags C-Fc-Avi
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Biotinylated Human B7-1 protein

ELISA: Immobilized Human CTLA-4-Fc at 5 ug/mL (100 uL/well) binds to Biotinylated Human B7-1 Protein-Fc-Avi with a linear range of 0.8-6 ng/mL.

ELISA: Serial dilutions of Ipilimumab were added into Human CTLA-4-Fc : Biotinylated Human B7-1 Protein-Fc-Avi binding reactions. The half maXimal inhibitory concentration (IC50) is 0.34215 ug/mL .


PRODUCT

  • SKU

    YCD1206-25ug

  • Size

    25ug

  • Price

    $525

1
  • SKU

    YCD1206-200ug

  • Size

    200ug

  • Price

    $2,070

1