Human FGF2 / FGF basic (Pro143-Ser288) protein, with N-Avi & N-10xHis Tag, expressed from E. coli

Home/ Products / Cytokines

PRODUCT

  • SKU

    YCD1271-25ug

  • Size

    25ug

  • Price

    $375

1
  • SKU

    YCD1271-200ug

  • Size

    200ug

  • Price

    $1,620

1
This recombinant protein contains Pro143-Ser288 of UniProt P09038. It has an Avi tag at the N-terminus, followed by a His tag. The protein's calculated molecular weight is 20.1 kDa, migrating at 20-22 kDa in reducing SDS-PAGE.
gene name FGF2
host species Human
production host E. coli
tags N-Avi & N-10xHis
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 50 mM Tris,150 mM NaCl,pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Biotinylated Human FGF basic protein

ELISA: Immobilized Human Glypican 3-Fc at 5 ug/mL (100 uL/well) binds to Biotinylated Human FGF basic-Avi-His with a linear range of 0.6-10 ng/mL.

ELISA: Immobilized Human FGFR2 (IIIb)-His at 5 ug/mL (100 uL/well) binds to Biotinylated Human FGF basic-Avi-His with a linear range of 0.01-0.313 ug/mL .


PRODUCT

  • SKU

    YCD1271-25ug

  • Size

    25ug

  • Price

    $375

1
  • SKU

    YCD1271-200ug

  • Size

    200ug

  • Price

    $1,620

1