Human ERBB2 / Her2 (Asn498-Ala648) protein, with C-10xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1282-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCD1282-1mg

  • Size

    1mg

  • Price

    $3,810

1
This recombinant protein contains Asn498-Ala648 of UniProt P04626. It has a His tag at the C-terminus. The protein's calculated molecular weight is 18.5 kDa, migrating at 28-35 kDa in reducing SDS-PAGE due to glycosylation. This protein has been verified not recognized by Pertuzumab in functional ELISA at ACRObiosystems.
gene name ERBB2
gene id UniProt P04626
host species Human
production host HEK293
tags C-10xHis
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human Her2 (498-648) protein

ELISA: Immobilized Human Her2 (498-648)-His at 1 ug/mL (100 uL/well) binds to Trastuzumab Biosimilar with a linear range of 0.1-2 ng/mL.

ELISA: Immobilized Human Her2 (498-648)-His at 2 ug/mL (100 uL/well) binds to Trastuzumab with a linear range of 0.2-1 ng/mL, This protein has been shown to weakly bind to Pertuzumab in our functional ELISA studies..


PRODUCT

  • SKU

    YCD1282-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCD1282-1mg

  • Size

    1mg

  • Price

    $3,810

1