Canine TNF / TNF-alpha (Val77-Leu233) protein, with C-6xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1342-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCD1342-1mg

  • Size

    1mg

  • Price

    $3,810

1
This recombinant protein contains Val77-Leu233 of UniProt P51742. It has a His tag at the C-terminus. The protein's calculated molecular weight is 18.6 kDa, migrating at 19-22 kDa in reducing SDS-PAGE due to glycosylation.
gene name TNF
host species Canine
production host HEK293
tags C-6xHis
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Canine TNF-alpha protein

ELISA: Immobilized Humira at 5 ug/mL (100 uL/well) binds to Canine TNF-alpha-His with a linear range of 0.3-1 ng/mL.

ELISA: Immobilized Human TNFR1-Fc at 5 ug/mL (100 uL/well) binds to Canine TNF-alpha-His with a linear range of 2-10 ng/mL .


PRODUCT

  • SKU

    YCD1342-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCD1342-1mg

  • Size

    1mg

  • Price

    $3,810

1