Human IL-35 heterodimer (EBI3 and IL12A ) protein, with C-Fc Tag, produced in HEK293

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PRODUCT

  • SKU

    YCD1495-100ug

  • Size

    100ug

  • Price

    $540

1
  • SKU

    YCD1495-1mg

  • Size

    1mg

  • Price

    $3,570

1
This recombinant protein contains Arg21-Lys229 (IL27beta, EBI3) of UniProt Q14213 and Arg23-Ser219 (IL12alpha, IL12A) of UniProt P29459. It has a human IgG1 Fc tag at the C-terminus. The protein's calculated molecular weight is 73.3 kDa, migrating at 80-90 kDa in reducing SDS-PAGE due to glycosylation.
gene name EBI3, IL12A
host species Human
production host HEK293
tags C-Fc
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human IL-35 protein

ELISA: Immobilized Human IL-35 R-His at 5 ug/mL (100 uL/well) binds to Human IL-35-Fc with a linear range of 0.002-0.625 ug/mL.


PRODUCT

  • SKU

    YCD1495-100ug

  • Size

    100ug

  • Price

    $540

1
  • SKU

    YCD1495-1mg

  • Size

    1mg

  • Price

    $3,570

1