Biotinylated human C5 / Complement C5 (Gln19-Cys1676) protein, with C-10xHis-Avi Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1538-25ug

  • Size

    25ug

  • Price

    $525

1
  • SKU

    YCD1538-200ug

  • Size

    200ug

  • Price

    $2,070

1
This recombinant protein contains Gln19-Cys1676 of UniProt P01031. It has a His tag at the C-terminus, followed by an Avi tag. The protein's calculated molecular weight is 189.9 kDa. As a result of glycosylation, the protein migrates as 75 kDa, 120 kDa and 200 kDa under reducing (R) condition, and 200 kDa under non-reducing (NR) condition (SDS-PAGE).
gene name C5
gene id UniProt P01031
host species Human
production host HEK293
tags C-10xHis-Avi
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Biotinylated Human Complement C5 protein

ELISA: Immobilized Biotinylated Human Complement C5-His-Avi at 1 ug/mL (100 uL/well) on streptavidin precoated (0.5 ug/well) plate binds to Monoclonal Anti-Human C5a Human Antibody with a linear range of 0.1-3 ng/mL.

ELISA: Immobilized Eculizumab Biosimilar at 2 ug/mL (100 uL/well) binds to Biotinylated Human Complement C5-His-Avi with a linear range of 1-63 ng/mL .


PRODUCT

  • SKU

    YCD1538-25ug

  • Size

    25ug

  • Price

    $525

1
  • SKU

    YCD1538-200ug

  • Size

    200ug

  • Price

    $2,070

1