HIV-1 GP140 Protein, withC-10xHis Tag, expressed from HEK293

Home/ Products / Viral Proteins

PRODUCT

  • SKU

    YCD1700-25ug

  • Size

    25ug

  • Price

    $825

1
  • SKU

    YCD1700-200ug

  • Size

    200ug

  • Price

    $3,300

1
This recombinant protein contains Ala30-Asp661 of UniProt Q2N0S5. It has a C-10xHis tag and was expressed from HEK293. The protein's calculated molecular weights are 53.7 kDa and 19.3 kDa, but migrates at 110-120 kDa under reducing conditions in SDS-PAGE due to glycosylation.
host species Human immunodeficiency virus 1 (HIV-1)
production host HEK293
tags C-10xHis
purity >90% as determined by SDS-PAGE.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation The protein was lyophilized from a solution of PBS, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human immunodeficiency virus 1 gp140 protein

ELISA: Immobilized Human CD4 Protein-Fc at 5 ug/mL (100 uL/well) binds to Human immunodeficiency virus 1 gp140 Protein-His with a linear range of 0.005-0.156 ug/mL.


PRODUCT

  • SKU

    YCD1700-25ug

  • Size

    25ug

  • Price

    $825

1
  • SKU

    YCD1700-200ug

  • Size

    200ug

  • Price

    $3,300

1