Human PCSK9 (Gln31-Gln692) protein, with C-10xHis Tag, expressed from HEK293

Home/ Products / ECD and Other Proteins

PRODUCT

  • SKU

    YCP1057-50ug

  • Size

    50ug

  • Price

    $525

1
  • SKU

    YCP1057-1mg

  • Size

    1mg

  • Price

    $3,795

1
This recombinant protein contains Gln31-Gln692 of UniProt Q8NBP7. It has a His tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non?covalent interactions and with a calculated MW of 13.8 kDa and 59.2 kDa respectively, migrating at 17 kDa and 65-70 kDa in reducing SDS-PAGE due to glycosylation.
gene name PCSK9
gene id UniProt Q8NBP7
host species Human
production host HEK293
tags C-10xHis
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human PCSK9 protein

ELISA: Immobilized Human PCSK9-His at 5 ug/mL (100 uL/well) binds to Human LDL R-Fc with a linear range of 10-156 ng/mL.

ELISA: Immobilized Human PCSK9-His at 2 ug/mL (100 uL/well) on an Nickel Coated plate binds to Human PCSK9 Mab (RN316) with a linear range of 0.2-3 ng/mL .


PRODUCT

  • SKU

    YCP1057-50ug

  • Size

    50ug

  • Price

    $525

1
  • SKU

    YCP1057-1mg

  • Size

    1mg

  • Price

    $3,795

1