|alias||FGF2, BFGF, FGFB, FGF basic, HBGF-2|
|production host||E. Coli|
|purity||>98% as determined by SDS-PAGE.|
|endotoxin||Less than 1.0 EU/ug by LAL method|
|formulation||Protein in production buffer (PBS, pH7.4) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.|
|reconstitution||Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.|
|shipping||Shipped at ambient conditions.|
|storage||Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.|
SDS-PAGE analysis of FGF2 protein
ELISA: Plate-coupled Human FGF basic, Tag Free at 1 ug/mL (100 uL/well) binds to Human Glypican 1 with a linear range of 1-39 ng/mL.
ELISA: Plate-coupled Human FGF basic, Tag Free at 2 ug/mL (100 uL/well) binds to Human Glypican 3 with a linear range of 0.48-7.8 ng/mL.