BenzNuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli. BenzNuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length.
★ Ultra Pure
★ Active in wide range of conditions
★ Removal of DNA/RNA from proteins
★ Purification of recombinant protein from inclusion bodies
★ Viscosity reduction of protein samples for downstream purification
|Shipped at ambient conditions.
|Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at below -20°C for up to 3 months.
SDS-PAGE analysis of BenzNuclease