HIV-1 [HIV-1/Clade B/C (CN54)] GP120 Protein, with C-6xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP1549-100ug

  • Size

    100ug

  • Price

    $390

1
  • SKU

    YCP1549-1mg

  • Size

    1mg

  • Price

    $2,970

1
This recombinant protein contains Thr36-Arg507 of UniProt G4XFJ5 (E46G, T396A, A497T). It has a C-6xHis tag and was expressed from HEK293. The protein's calculated molecular weight is 53.8 kDa, but migrates at 85-150 kDa under reducing conditions in SDS-PAGE due to glycosylation.
host species HIV-1 (CN54)
production host HEK293
tags C-6xHis
purity >95% as determined by SDS-PAGE.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation The protein was lyophilized from a solution of PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of HIV-1 [HIV-1/Clade B/C (CN54)] GP120 protein

ELISA: Immobilized HIV-1 [HIV-1/Clade B/C (CN54)] GP120-His at 5 ug/mL (100 uL/well) binds to Human CD4-Fc with a linear range of 1-39 ng/mL.


PRODUCT

  • SKU

    YCP1549-100ug

  • Size

    100ug

  • Price

    $390

1
  • SKU

    YCP1549-1mg

  • Size

    1mg

  • Price

    $2,970

1