|alias||PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1, PDL1|
|purity||>95% as determined by SDS-PAGE.|
|endotoxin||The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 0.1 EU/ug).|
|formulation||Protein in production buffer (PBS, pH7.4) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.|
|reconstitution||Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.|
|shipping||Shipped at ambient conditions.|
|storage||Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.|
SDS-PAGE analysis of CD274 protein
ELISA: Plate-coupled Human PD-L1 at 5 ug/mL (100 uL/well) binds to Anti-PD-L1 MAb with a linear range of 0.2-2 ng/mL.
ELISA: Plate-coupled Human PD-1, Strep Tag at 2 ug/mL (100 uL/well) binds to Human PD-L1 with a linear range of 4-63 ng/mL.
SEC-HPLC analysis shows protein purity > 90%