Human CD80 / B7-1 (Val35-Asn242) protein, with C-Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP2013-100ug

  • Size

    100ug

  • Price

    $390

1
  • SKU

    YCP2013-1mg

  • Size

    1mg

  • Price

    $2,910

1
This recombinant protein contains Val35-Asn242 of UniProt P33681. It has a human IgG1 Fc tag at the C-terminus. The protein's calculated molecular weight is 50.0 kDa, migrating at 65-90 kDa in reducing SDS-PAGE due to glycosylation.
gene name CD80
gene id UniProt P33681
host species Human
production host HEK293
tags C-Fc
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 50 mM Tris, 100 mM Glycine, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human B7-1 protein

ELISA: Immobilized Human B7-1-Fc at 2 ug/mL (100 uL/well) binds to Human CTLA-4-His with a linear range of 0.1-2 ng/mL.

ELISA: Immobilized Human B7-1-Fc at 5 ug/mL (100 uL/well) binds to Biotinylated Human PD-L1-Fc-Avi,His Tag with a linear range of 0.02-1.25 ug/mL .


PRODUCT

  • SKU

    YCP2013-100ug

  • Size

    100ug

  • Price

    $390

1
  • SKU

    YCP2013-1mg

  • Size

    1mg

  • Price

    $2,910

1