Human CD274 / PD-L1 (Phe19-Arg238) protein, with C-mFc (mIgG1) Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5737-100ug

  • Size

    100ug

  • Price

    $405

1
  • SKU

    YCP5737-1mg

  • Size

    1mg

  • Price

    $2,445

1
This recombinant protein contains Phe19-Arg238 of UniProt Q9EP73. It has a mouse IgG1 Fc tag at the C-terminus. The protein's calculated molecular weight is 51.4 kDa, migrating at 55-65 kDa in reducing SDS-PAGE due to glycosylation.
gene name CD274
gene id UniProt Q9EP73
host species Human
production host HEK293
tags C-mFc (mIgG1)
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human PD-L1 protein

ELISA: Immobilized Human PD-1, Strep Tag at 1 ug/mL (100 uL/well) binds to Human PD-L1 Protein, Mouse IgG1 Fc Tag with a linear range of 5-78 ng/mL.

ELISA: Serial dilutions of Nivolumab were added into Human PD-L1 Protein, Mouse IgG1 Fc Tag : Human PD-1, Strep Tag binding reactions. The half maXimal inhibitory concentration (IC50) is 0.2391 ug/mL.


PRODUCT

  • SKU

    YCP5737-100ug

  • Size

    100ug

  • Price

    $405

1
  • SKU

    YCP5737-1mg

  • Size

    1mg

  • Price

    $2,445

1